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KPDC, Korea Polar Data Center

Korea Polar Data Center Scientific observations and results from Antarctica shall
be exchanged and made freely available

trimMSmix: a software to debarcode MiSeq sequences

trimMSmix is a python script to trim priming regions, decode according to barcodes, and assemble forward and reverse sequences generated by MiSeq. It handles sequences saved in two files for forward and reverse sequences. Sequence files contain sequences for diverse PCR amplicons produced by diverse primers with barcode. It recognizes primer sequences first to sort different genetic loci and then barcode to sort different samples.

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Entry ID
DOI
https://dx.doi.org/doi:10.22663/KOPRI-KPDC-00002438.1
Copyright
Personnel
  • Soon Gyu Hong (polypore@kopri.re.kr)
Create/Update Date
2023-12-27 / 2023-12-27
Citation
The data(KOPRI-KPDC-00002438) used in this work was provided by the Korea Polar Research Institute.

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File Size 279.1 Kb for 4 items
Category File Name Description Size Status
Rawdata Manual_trimMSmix.pdf manual for trimMSmix 35.77 Kb Download
Rawdata trimInfo_example.txt trimInfo example text file 2.36 Kb Download
Rawdata trimInfo_example.xlsx trimInfo template example Excel file 212.15 Kb Download
Rawdata trimMSmix.pyc trimMSmix: a python script 28.81 Kb Download