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KPDC, Korea Polar Data Center

Korea Polar Data Center Scientific observations and results from Antarctica shall
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X-ray diffraction data of GerE

In cold and harsh environments such as glaciers and sediments in ice cores, microbes can survive by forming spores. Spores are composed of a thick coat protein, which protects against external factors such as heat-shock, high salinity, and nutrient deficiency. GerE is a key transcription factor involved in spore coat protein expression in the mother cell during sporulation. GerE regulates transcription during the late sporulation stage by directly binding to the promoter of cotB gene. Here, we report the crystal structure of PaGerE at 2.09 ? resolution from Paenisporosarcina sp. TG-14, which was isolated from the Taylor glacier. The PaGerE structure is composed of four α-helices and adopts a helix-turn-helix architecture with 68 amino acid residues. Based on our DNA binding analysis, the PaGerE binds to the promoter region of CotB to affect protein expression. Additionally, our structural comparison studies suggest that DNA binding by PaGerE causes a conformational change in the α4-helix region, which may strongly induce dimerization of PaGerE.

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Entry ID
KOPRI-KPDC-00001212
DOI
https://dx.doi.org/doi:10.22663/KOPRI-KPDC-00001212.1
Science Keyword
EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS
ISO Topic
Biota
Personnel
Changwoo Lee (justay@kopri.re.kr)
Jun Hyuck Lee (junhyucklee@kopri.re.kr)
Project
PE19210, Development of potential candidates as antibiotics based on polar genetic resources. PI.Lee, Jun Hyuck
Research period
2015-10-15 - 2015-10-15
Create/Update Date
2019-09-30 08:24:01 UTC
Location
CONTINENT > ASIA > EASTERN ASIA > SOUTH KOREA > BL-5C of the Pohang Accelerator Laboratory
Raw Data
File Size: 3.47 Mb for 1 item
Citation
The data(KOPRI-KPDC-00001212) used in this work was provided by the Korea Polar Research Institute.
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  • [lat:36°01'24.6"N, lon:129°18'58.8"E]

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